Composite

Part:BBa_K2753022:Design

Designed by: WEI KUANGYI   Group: iGEM18_GreatBay_China   (2018-10-01)


TALE2 sp5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1513
    Illegal NheI site found at 1921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 113
    Illegal XhoI site found at 2152
    Illegal XhoI site found at 2692
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 747
    Illegal AgeI site found at 849
    Illegal AgeI site found at 1767
    Illegal AgeI site found at 2277
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

Synthesis from company

References

Segall-shapiro, T. H., Sontag, E. D., & Voigt, C. A. (2018). Engineered promoters enable constant gene expression at any copy number in bacteria, (March). https://doi.org/10.1038/nbt.4111